Dion is a village in Pieria prefecture, 440 km from Athens and 85 km from Thessaloniki. Excavations not far from the village unearthed a fortified city that had been inhabited continuously from the sixth century bc to the fifth century ad. The museum opened in 1983 in a newly-constructed two-storey building. It displays finds mainly from the Dion area, but also from Olympus and the wider area of Pieria (on the first floor).

The display includes statues, votive and grave monuments, architectural members, coins, and a variety of other objects that were discovered in the sanctuaries, the baths, and the necropolis, as also objects used in the everyday life of the ancient city of Dion. The finds are grouped according to the area and the specific places where they were found.

On the ground floor, visitors may admire statues of Dionysos, the family of Asklepios and other statuary from the baths, the devotional statues of Isis and Aphrodite Hypolympia, together with other votive statues from the Sanctuary of Isis, and the heads of Demetrios, from the sanctuary of that name, and Aphrodite, from her altar. There are a number of exhibits from the necropolis, mainly wooden figurines, and other votive offerings found in Macedonian tombs. There are also display cases containing architectural members and other objects from Early Christian basilicas, and a large collection of coins.

On the upper floor are objects used in the day-to-day life of the people of Dion, together with devotional artefacts and statues from all over Pieria. A special room on this floor contains a hydraulis or water organ, the first organ found in Greece and the oldest anywhere in the world. It was described by Hero of Alexandria and Vitruvius.

The museum has a special room for educational games and guided tours for junior-school children, as also a film room.